pcdna3 1 neo expression vector (Thermo Fisher)
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pcdna3 1 neo expression vector
Pcdna3 1 Neo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+neo+expression+vector/us11312757-1416-15-18
Average 86 stars, based on 1 article reviews
Pcdna3 1 Neo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+neo+expression+vector/us11312757-1416-15-18
Average 86 stars, based on 1 article reviews
pcdna3 1 neo expression vector - by Bioz Stars,
2026-09
86/100 stars
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Amplification:Article Title: Sorting Protein-related Receptor SorLA Controls Regulated Secretion of Glial Cell Line-derived Neurotrophic Factor Article Snippet: The other antibodies were purchased as follows: mouse anti-GDNF, rabbit anti-SorLA, and goat anti-RET antibodies from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-phosphotyrosine antibody 4G10 from Upstate Laboratories (Charlottesville, VA); anti-sortilin antibodies from BD Biosciences (Franklin Lakes, NJ); mouse anti-Akt, rabbit anti-phospho-AktS473, rabbit anti-p44/42 MAP kinase (ERK1/2), mouse anti-phospho-p44/42 (pERK1/2) (Thr202/Tyr204) antibodies from Cell Signaling Technology (Beverly, MA); Alexa Fluor 488 goat anti-mouse IgG (H+L), Alexa Fluor 594 goat anti-rabbit IgG (H+L) from Invitrogen (Carlsbad, CA), and Cy5-conjugated donkey anti-rabbit IgG (H+L) from Jackson ImmunoResearch Laboratories (West Grove, PA). .. The human full-length (FL) GDNF and GDNFΔ78 cDNA were amplified by RT-PCR from human brain tissue and subcloned into Article Title: Troglitazone, a Peroxisome Proliferator-activated Receptor γ (PPARγ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARγ Article Snippet: Construction of Plasmids—The full-length Egr cDNAs were generated by PCR from human universal QUICK-Clone cDNA (Clontech, Palo Alto, CA) using the following primers: for the Egr-1, 5 -GACACCAGCTCTCCAGCCTGCTCGTCCAGG-3 (top strand) and 5 -TTCCCTTTAGCAAATTTCAATTGTCCTGGG-3 (bottom strand); for the Egr-2, 5 -GTGCGAGGAGCAAATGATGACCGCCAAGGC-3 (top strand) and 5 -CAGCCTGAGTCTCATCTCAAGGTGTCCGGG-3 (bottom strand); for the Egr-3, 5 -CGGCGGCAGCTCGGGAGTGCTATGACCGGC-3 (top strand) and 5 -TCTGGGGGCCCGATCCTCAGGCGCAGGTGG-3 (bottom strand). .. The amplified products were cloned into pCR2.1 TOPO vector (Stratagene, CA) and followed by cloning into Article Title: Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin Article Snippet: The 100% Percoll gradient was made by adding 10 ml of 10X RPMI-1640 containing 200 mM HEPES/ 1M NaOH to 90 mL of commercial Percoll solution (GE Healthcare, Piscataway, NJ). .. The human NDST2 gene (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"BC035711.1","term_id":"23243098","term_text":"BC035711.1"}} BC035711.1 , Thermo Fisher Scientific) was amplified using a primer pair, which includes BamHI and NotI restriction enzyme sites (forward primer: 5`-GAGCTC GGATCC ACTATGCTCCAGTTGTGGAAGGTG-3`; reverse primer: 5`-CTCGA GCGGCCGC TCAGCCCAGACTGGAATGCTG-3`) and inserted into the Reverse Transcription Polymerase Chain Reaction:Article Title: Sorting Protein-related Receptor SorLA Controls Regulated Secretion of Glial Cell Line-derived Neurotrophic Factor Article Snippet: The other antibodies were purchased as follows: mouse anti-GDNF, rabbit anti-SorLA, and goat anti-RET antibodies from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-phosphotyrosine antibody 4G10 from Upstate Laboratories (Charlottesville, VA); anti-sortilin antibodies from BD Biosciences (Franklin Lakes, NJ); mouse anti-Akt, rabbit anti-phospho-AktS473, rabbit anti-p44/42 MAP kinase (ERK1/2), mouse anti-phospho-p44/42 (pERK1/2) (Thr202/Tyr204) antibodies from Cell Signaling Technology (Beverly, MA); Alexa Fluor 488 goat anti-mouse IgG (H+L), Alexa Fluor 594 goat anti-rabbit IgG (H+L) from Invitrogen (Carlsbad, CA), and Cy5-conjugated donkey anti-rabbit IgG (H+L) from Jackson ImmunoResearch Laboratories (West Grove, PA). .. The human full-length (FL) GDNF and GDNFΔ78 cDNA were amplified by RT-PCR from human brain tissue and subcloned into Expressing:Article Title: Sorting Protein-related Receptor SorLA Controls Regulated Secretion of Glial Cell Line-derived Neurotrophic Factor Article Snippet: The other antibodies were purchased as follows: mouse anti-GDNF, rabbit anti-SorLA, and goat anti-RET antibodies from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-phosphotyrosine antibody 4G10 from Upstate Laboratories (Charlottesville, VA); anti-sortilin antibodies from BD Biosciences (Franklin Lakes, NJ); mouse anti-Akt, rabbit anti-phospho-AktS473, rabbit anti-p44/42 MAP kinase (ERK1/2), mouse anti-phospho-p44/42 (pERK1/2) (Thr202/Tyr204) antibodies from Cell Signaling Technology (Beverly, MA); Alexa Fluor 488 goat anti-mouse IgG (H+L), Alexa Fluor 594 goat anti-rabbit IgG (H+L) from Invitrogen (Carlsbad, CA), and Cy5-conjugated donkey anti-rabbit IgG (H+L) from Jackson ImmunoResearch Laboratories (West Grove, PA). .. The human full-length (FL) GDNF and GDNFΔ78 cDNA were amplified by RT-PCR from human brain tissue and subcloned into Article Title: Troglitazone, a Peroxisome Proliferator-activated Receptor γ (PPARγ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARγ Article Snippet: Construction of Plasmids—The full-length Egr cDNAs were generated by PCR from human universal QUICK-Clone cDNA (Clontech, Palo Alto, CA) using the following primers: for the Egr-1, 5 -GACACCAGCTCTCCAGCCTGCTCGTCCAGG-3 (top strand) and 5 -TTCCCTTTAGCAAATTTCAATTGTCCTGGG-3 (bottom strand); for the Egr-2, 5 -GTGCGAGGAGCAAATGATGACCGCCAAGGC-3 (top strand) and 5 -CAGCCTGAGTCTCATCTCAAGGTGTCCGGG-3 (bottom strand); for the Egr-3, 5 -CGGCGGCAGCTCGGGAGTGCTATGACCGGC-3 (top strand) and 5 -TCTGGGGGCCCGATCCTCAGGCGCAGGTGG-3 (bottom strand). .. The amplified products were cloned into pCR2.1 TOPO vector (Stratagene, CA) and followed by cloning into Article Title: Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin Article Snippet: The 100% Percoll gradient was made by adding 10 ml of 10X RPMI-1640 containing 200 mM HEPES/ 1M NaOH to 90 mL of commercial Percoll solution (GE Healthcare, Piscataway, NJ). .. The human NDST2 gene (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"BC035711.1","term_id":"23243098","term_text":"BC035711.1"}} BC035711.1 , Thermo Fisher Scientific) was amplified using a primer pair, which includes BamHI and NotI restriction enzyme sites (forward primer: 5`-GAGCTC GGATCC ACTATGCTCCAGTTGTGGAAGGTG-3`; reverse primer: 5`-CTCGA GCGGCCGC TCAGCCCAGACTGGAATGCTG-3`) and inserted into the Article Title: Fusion protein comprising IL13 Article Snippet: .. Drugs and administration: The IL4/IL13 was produced by transient transfection of HEK293F cells with the Plasmid Preparation:Article Title: Sorting Protein-related Receptor SorLA Controls Regulated Secretion of Glial Cell Line-derived Neurotrophic Factor Article Snippet: The other antibodies were purchased as follows: mouse anti-GDNF, rabbit anti-SorLA, and goat anti-RET antibodies from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-phosphotyrosine antibody 4G10 from Upstate Laboratories (Charlottesville, VA); anti-sortilin antibodies from BD Biosciences (Franklin Lakes, NJ); mouse anti-Akt, rabbit anti-phospho-AktS473, rabbit anti-p44/42 MAP kinase (ERK1/2), mouse anti-phospho-p44/42 (pERK1/2) (Thr202/Tyr204) antibodies from Cell Signaling Technology (Beverly, MA); Alexa Fluor 488 goat anti-mouse IgG (H+L), Alexa Fluor 594 goat anti-rabbit IgG (H+L) from Invitrogen (Carlsbad, CA), and Cy5-conjugated donkey anti-rabbit IgG (H+L) from Jackson ImmunoResearch Laboratories (West Grove, PA). .. The human full-length (FL) GDNF and GDNFΔ78 cDNA were amplified by RT-PCR from human brain tissue and subcloned into Article Title: Troglitazone, a Peroxisome Proliferator-activated Receptor γ (PPARγ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARγ Article Snippet: Construction of Plasmids—The full-length Egr cDNAs were generated by PCR from human universal QUICK-Clone cDNA (Clontech, Palo Alto, CA) using the following primers: for the Egr-1, 5 -GACACCAGCTCTCCAGCCTGCTCGTCCAGG-3 (top strand) and 5 -TTCCCTTTAGCAAATTTCAATTGTCCTGGG-3 (bottom strand); for the Egr-2, 5 -GTGCGAGGAGCAAATGATGACCGCCAAGGC-3 (top strand) and 5 -CAGCCTGAGTCTCATCTCAAGGTGTCCGGG-3 (bottom strand); for the Egr-3, 5 -CGGCGGCAGCTCGGGAGTGCTATGACCGGC-3 (top strand) and 5 -TCTGGGGGCCCGATCCTCAGGCGCAGGTGG-3 (bottom strand). .. The amplified products were cloned into pCR2.1 TOPO vector (Stratagene, CA) and followed by cloning into Article Title: Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin Article Snippet: The 100% Percoll gradient was made by adding 10 ml of 10X RPMI-1640 containing 200 mM HEPES/ 1M NaOH to 90 mL of commercial Percoll solution (GE Healthcare, Piscataway, NJ). .. The human NDST2 gene (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"BC035711.1","term_id":"23243098","term_text":"BC035711.1"}} BC035711.1 , Thermo Fisher Scientific) was amplified using a primer pair, which includes BamHI and NotI restriction enzyme sites (forward primer: 5`-GAGCTC GGATCC ACTATGCTCCAGTTGTGGAAGGTG-3`; reverse primer: 5`-CTCGA GCGGCCGC TCAGCCCAGACTGGAATGCTG-3`) and inserted into the Clone Assay:Article Title: Troglitazone, a Peroxisome Proliferator-activated Receptor γ (PPARγ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARγ Article Snippet: Construction of Plasmids—The full-length Egr cDNAs were generated by PCR from human universal QUICK-Clone cDNA (Clontech, Palo Alto, CA) using the following primers: for the Egr-1, 5 -GACACCAGCTCTCCAGCCTGCTCGTCCAGG-3 (top strand) and 5 -TTCCCTTTAGCAAATTTCAATTGTCCTGGG-3 (bottom strand); for the Egr-2, 5 -GTGCGAGGAGCAAATGATGACCGCCAAGGC-3 (top strand) and 5 -CAGCCTGAGTCTCATCTCAAGGTGTCCGGG-3 (bottom strand); for the Egr-3, 5 -CGGCGGCAGCTCGGGAGTGCTATGACCGGC-3 (top strand) and 5 -TCTGGGGGCCCGATCCTCAGGCGCAGGTGG-3 (bottom strand). .. The amplified products were cloned into pCR2.1 TOPO vector (Stratagene, CA) and followed by cloning into Cloning:Article Title: Troglitazone, a Peroxisome Proliferator-activated Receptor γ (PPARγ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARγ Article Snippet: Construction of Plasmids—The full-length Egr cDNAs were generated by PCR from human universal QUICK-Clone cDNA (Clontech, Palo Alto, CA) using the following primers: for the Egr-1, 5 -GACACCAGCTCTCCAGCCTGCTCGTCCAGG-3 (top strand) and 5 -TTCCCTTTAGCAAATTTCAATTGTCCTGGG-3 (bottom strand); for the Egr-2, 5 -GTGCGAGGAGCAAATGATGACCGCCAAGGC-3 (top strand) and 5 -CAGCCTGAGTCTCATCTCAAGGTGTCCGGG-3 (bottom strand); for the Egr-3, 5 -CGGCGGCAGCTCGGGAGTGCTATGACCGGC-3 (top strand) and 5 -TCTGGGGGCCCGATCCTCAGGCGCAGGTGG-3 (bottom strand). .. The amplified products were cloned into pCR2.1 TOPO vector (Stratagene, CA) and followed by cloning into Produced:Article Title: Fusion protein comprising IL13 Article Snippet: .. Drugs and administration: The IL4/IL13 was produced by transient transfection of HEK293F cells with the Transfection:Article Title: Fusion protein comprising IL13 Article Snippet: .. Drugs and administration: The IL4/IL13 was produced by transient transfection of HEK293F cells with the |